Krameria lappacea (Rhatany or Ratanhia, Krameriaceae) is an endangered, overexploited medicinal plant from the semi-deserts of Andean. Description and images of Krameria lappacea (), a native Chilean plant, provided by the supplier of native exotic Chilean seeds and organizer of plant watching. Krameria lappacea (Rhatany or Ratanhia, Krameriaceae) is an endangered, overexploited medicinal plant from the semi-deserts of Andean South America, and.

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Krameria lappacea – Wikipedia

The content of the lignan derivatives in the tincture samples 2—4 was very consistent with the highest variation for compound 7 0. Author information Article notes Copyright and License information Disclaimer.

This validated assay will be a useful tool for quality control of the plant material and its preparations, focusing on the compound class of lignan derivatives. National Center for Biotechnology InformationU. Other traditional indications are diarrhoea and mouth ulcer [1].

Associated Data Supplementary Materials mmc1. In the course of the present work compound 7, one additional prominent peak, was isolated out of the roots of K. Suitability of the developed method for the quantification of the major lignan derivatives in Ratanhiae radix and Tinctura Ratanhiae can be deduced from several analytical parameters.

Compounds 1 — 6 and 8 — 12 were obtained and identified in a previous study as:.

Krameria lappacea Images – Useful Tropical Plants

The methanol insoluble part The flowers have glands called elaiophores which produce a lipid which is collected by bees of the genus Centris as they pollinate the flowers.

For quantification of compound 8 and 11 the obtained solutions were further diluted with methanol 1: This article has been cited by other articles in PMC. ESI, alternating mode; spray voltage: Krameria are found across the Americaswith most native to the tropical regions.

Moreover it was obvious that by using just methanol or acetonitrile alone no satisfactory separation was achieved. In order to check if this extraction protocol is exhaustive, another 5 cycles after the normal extraction protocol were conducted and the obtained extract analyzed by HPLC.


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Support Center Support Center. Five additional calibration levels were prepared by dilution with methanol, lappzcea each level was assayed in triplicate see Table 1 for calibration data. Rhatany is also the name given to krameria roota botanical remedy consisting of the dried root of para rhatany Krameria argentea or Peruvian rhatany Krameria lappacea.

The initial screening experiments stationary phases with RP, RP, ether-linked phenyl, phenyl-hexyl column material clearly showed that the stationary phase kraameria to be a phenyl-hexyl column, since this phase exclusively enabled the baseline separation of ratanhiaphenols II 11 and I 12which represent the most prominent constituents in Ratanhiae radix. Appendix A Supplementary data associated with this article can be found, in the online version, at doi: This issue could be explained by the shorter maceration time 3 dayssince regular Tinctura Ratanhiae is prepared by a double maceration over a period of two weeks.

According to solubility different starting concentrations were used: Separation was achieved on a phenyl-hexyl column material using a solvent gradient consisting of 0. Results and discussion 3. Accuracy was determined in sample RR-1 as well as in sample T-1 T-2 in case of compound 4 lappaceaa spiking experiments at two different concentrations. Besides gravimetrical analysis of the ratanhiaphenols I, II oappacea III, the content of these compounds in the herbal drug has never been determined.

In order to accomplish a satisfactory separation, all parameters had to be carefully assessed. Several mixtures thereof This page was last edited on 21 Novemberat Following these conditions the lignan derivatives were exhaustively extracted but the yield of undesired tannins could be minimized. Highest deviations within the root samples were found for compounds 5 and 12 with contents ranging from 0. Isolation and structure determination of new active neolignans and norneolignans from Ratanhia.

For an exhaustive extraction the procedure was carried out twice.

All other parameters were maintained. Together with the fact that all compounds, except the stereoisomers 2 and 3were baseline separated, selectivity of the assay is indicated. Supplementary data Click here to view.

Method development The major problem in the development of a Karmeria method for the simultaneous detection of all active lignan derivatives was the structural similarity of the analytes.


The topical anti-inflammatory properties of the secondary metabolites were comparable to the drug indomethacin ID 50 value 0. By mass spectrometry, 1- and 2D-NMR experiments and comparison with published data [7] this lignan derivative was identified as 3-formyl 4-hydroxyphenyl E -propenylbenzofuran. In the case of root samples, different solvents methanol, acetone, DCM and mixtures thereof and extraction procedures sonication, ASE extraction were evaluated.

Abstract Root preparations of Krameria lappacea Dombey Burdet krzmeria Simpson are traditionally used kramerix oropharyngeal inflammation. Thus, the HPLC-method, developed in the present study, is a significant improvement and innovation, krameri it enables for the first time the quantitative analysis of lignan derivatives in Ratanhiae radix as well as in Tinctura Ratanhiae. Journal of Pharmaceutical and Biomedical Analysis.

Compounds 1 — 6and 8 — 12 were isolated and their structures elucidated in a previous study [10]. Retrieved from ” https: The content of these compounds in the drug, besides a gravimetrical analysis of the ratanhiaphenols I, II and III content of 0.

In a previous study we could show that not only tannins with their astringent and antimicrobial properties, but also the neglected compound class of lignan derivatives with their pronounced anti-inflammatory activities play an lappcea role in the use of the herbal drug against inflammatory disorders of mouth and throat.

Therefore we developed an efficient and according to the ICH guidelines validated HPLC method for the quantification of the active constituents in the roots as well as in the ethanolic tincture. Quality of root samples of K.

Precision of the method was determined by preparing and assaying 5 replicate samples of RR-1 and T-1 on the same day; the same procedure was repeated on two more days.